P-278 What are the effects of ICSI procedure time intervals on in vitro fertilization outcomes?

Abstract Study question Do time intervals between oocyte pick-up (OPU), denudation (OD), and Intra-cytoplasmic Sperm Injection (ICSI) impact ICSI outcomes? Summary answer maturation, blastulation pregnancy rates. There is no effect on fertilization cleavage What known already Cytoplasmic nuclear maturation are crucial for the success of in vitro embryo development. Cumulus cells enhance maturation. Therefore, prolonged culture prior to may induce apoptosis. consensus optimal different procedures their effects fertilization, blastocyst, development design, size, duration A retrospective analysis 794 fresh cycles was performed at Alyssa Fertility Group Center, Juin 2021 December 2022. Participants/materials, setting, methods were women aged ≤40 years, with least 3 mature oocytes. Semen partner concentrations greater than 1million/mL included. Time OPU, OD, recorded. We evaluate these timing Statistical using SPSS 22.0. Kolmogorov–Smirnov, Student t, Pearson tests, ROC curve used. Level signification p-value <0.05. Main results role chance The mean women’s age 33,2 years (SD 4,4). Mean times hours OPU/OD, OD/ICSI, OPU/ respectively: 01:28(SD 00:37); 02:35(SD 01:01); 04:03(SD 01:08). Positive correlations seen rate OPU/OD; OPU/ICSI respectively (r = 0,217, p 0,002; r 0,193, 0,005).There significant correlation intervals, Top quality embryos rates positively associated OPU/OD interval 0,136: 0,049). respectively(r 0,183: 0,008; 0,211: 0,279: < 0,001).Pregnancy correlated OD/ICSI 0,158: 0,022; 0,188: 0,006). curves revealed that cut off value between: 01:10 (AUC 0,554; IC 95% 0,476-0,632), 0,185; 02:20 0,600; 0,516-0,669), 0,023 OPU/ICSI: 03:50, 0,524-0,676), 0,014. Limitations, reasons caution Trigger an important factor should be noted. This study limited by its nature. Potential unmeasured confounding cannot excluded Wider implications findings all affect reproductive outcomes. According our findings, excessive incubation before avoided. 2 after OD. adjusted according laboratory practice improve Trial registration number Not applicable